Analysis of the biocompatibility of a biocelulose and a poly L- lactic acid membrane

The use of selective barriers as resorbable membranes has become a routine clinical procedure for guided bone regeneration. Therefore, the production of membranes with a low inflammatory potential during their resorption process has become the goal of a considerable number of researches. Aim: The purpose of the present study was to evaluate the biocompatibility of poly (L- lactic acid) (PLLA) and biocelulose membranes (BC) inserted in the subcutaneous tissue on the dorsum of rats. Methods: Fifteen animals underwent surgical procedures for the insertion of 4 types of membranes: COL (Collagen membrane) ­ Control Group; BC (Biocellulose membrane); BCAg (Biocellulose membrane impregnated with Silver); PLLA (Poly (L-lactic acid) membrane). All membrane types were inserted into each animal. Animals were euthanized after 3, 7, and 15 days of the surgical procedure. Descriptive histological analyses were carried out to investigate host tissue reaction to membrane presence by assessing the anti-inflammatory process composition associated with the membrane resorption and the presence of foreign-body reaction or encapsulation. Results: The BC membranes showed a higher degree of inflammation and poor pattern of integration with the surrounding tissues than the PLLA and COL membranes. Conclusion: The PLLA and COL membranes present better biocompatibility than the BC membranes

Health literacy of mothers on factors associated to pre-rupture of membranes on perinatal outcomes in Vhembe district, South Africa

Pre-rupture of membranes prior labour initiation could complicate approximately 5% of pregnancies and could be associated with a high incidence of perinatal morbidity and mortality complications. The major complications could be chorioamnionitis and cord compression resulting in hypoxia. This study aimed to assess the health literacy of mothers on perinatal outcomes following prerupture of membranes at Thulamela B clinics of Vhembe district. A quantitative research approach with a cross-sectional descriptive design was used. The study population comprised a total of 210 lactating mothers within 6 weeks following delivery,irrespective of the mode of delivery were purposively selected. Data were collected by the researchers through self-administered questionnaires. The Statistical Package for Social Sciences (SPSS) version 25 was used for data analysis. The results are presented in the form of tables and graphs based on probability allowing judgment to be made on variables. Validity and reliability were ensured. Ethical clearance was obtained from the University of Venda Research Ethics Committee and permission to access health facilities from the Limpopo Province Department of Health. Findings revealed that limited and inadequate health literacy towards pre-rupture of membranes contributes to perinatal morbidity and mortality, with associated factors like poor antenatal care attendance, delayed health seeking, defaulting, and loss of follow-up. Recommendations emphasise the provision of preconception and antenatal care where health information on risks and danger signs of PROM to perinatal outcomes are given were made. (Afr J Reprod Health 2022; 26[7]: 90-101).

Bioactivities of Alchemilla mollis, Alchemilla persica and Their Active Constituents

Abstract The aim of this study was to determine antimicrobial activities of Alchemilla mollis, Alchemilla persica as well as ellagic acid and miquelianin against Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans by using microbroth dilution method and anti-inflammatory activity by using human red blood cell (HRBC) membrane stabilization method. Microbroth dilution method was used to determine the antimicrobial activities. Extracts possessed activity having MIC values of 2.5-5-10mg/ mL, compounds possessed activity having MIC values of 1.25-2.5-4-5mg/mL. A.mollis aerial parts displayed the highest anti-inflammatory activity (IC50=1.22±0.07mg/mL). Ellagic acid and miquelianin were also determined as anti-inflammatory agents with 0.57±0.01mg/mL and 1.23±0.02mg/mL IC50 values, respectively. Total phenolic content and tannin content of the A.mollis and A.persica were determined as 357.00±75.80mg, 282.50±28.70mg PGE/g plant material and 18.02%, 18.63% respectively according to the method described by European Pharmacopoeia. Ellagic acid, miquelianin and catechin were analyzed by HPLC. The highest catechin content was detected in A.persica roots (6.69±0.05g/100g plant material). A.mollis aerial parts contain higher miquelianin (0.39±0.02g/plant material) and ellagic acid (1.56±0.01g/ plant material) than A.persica.

Avaliação da membrana de colágeno de peixe na regeneração óssea guiada: estudo histológico, histomorfométrico, imunoistoquímico, micro tomográfico em calotas de ratos / Evaluation of fish collagen membrane in guided bone regeneration: histological, histomorphometric, immunohistochemical, micro tomographic study on ice caps rats

O processo de regeneração óssea guiada é amplamente conhecido na literatura, e para que essa técnica possa ser realizada com sucesso a escolha da barreira biológica no tocante ao seu tempo de degradação e sua reação tecidual é de fundamental importância. Assim, o objetivo deste estudo foi avaliar o processo de reparo de defeitos ósseos críticos em calotas de ratos, utilizando o princípio de osteopromoção, comparando a membrana teste de colágeno de peixe funcionalizando com hidroxiapatita com as membrana de colágeno bovino associada à hidroxiapatita e a membrana de colágeno porcino. Para o estudo foram utilizados 96 ratos Albinus Wistar, divididos em quatro grupos, sendo 24 animais para cada grupo: Grupos BG (BioGide®); CB (ColHap®); CP (colágeno de peixe) e CG (Coágulo) analisados em quatro tempos experimentais, 7, 15, 30 e 60 dias. Os resultados mostraram um perfil inflamatório maior no grupo CP em relação aos grupos BG e CB (p< 0,05) nos primeiros 15 dias. O grupo CP, aos 60 dias apresentou um bom potencial osteopromotor ao compará-lo com o grupo BG (p = 0,199) e o grupo CB demonstrou o menor desempenho osteopromotor. Na análise tridimensional, os resultados anteriores foram confirmados com o pior desempenho em relação a menor média detecido ósseo neoformado para o grupo CP de 9,560mm3 , CB com 11,850mm3 e BG 14,247mm3 (p< 0,05). Concluí-se que as membranas aqui estudadas tiveram um bom comportamento biológico, e todas auxiliaram no processo de regeneração óssea guiada e que a membrana de colágeno de peixe teve um bom desempenho na osteopromoção óssea(AU)

Guided bone regeneration is a widely described alveolar ridge augmentation technique that has been shown to produce excellent and reproducible results with high long-term success rates in several studies. Thus, this study aimed to evaluate the bone repair of critical size defects calavaria using an osteopromotive principle the fish collagen membrane functionalized with hydroxyapatite compare to collagen membrane associated with hydroxyapatite and porcine membrane. For the study, 96 Albinus Wistar rats were used, divided into four groups, with 24 animals for each group: Groups BG (BioGide®); CB (ColHap®); CP (fish collagen) and CG (clot) analyzed in four experimental times, 7, 15, 30 and 60 days. The results showed a higher inflammatory profile in the CP group compared to the BG and CB groups (p< 0.05) in the first 15 days after the surgery. The CP group, at 60 days, showed a good osteopromotor potential when compared with the BG group (p = 0.199) and the CB group showed the smaller osteopromotor performance. In the three-dimensional analysis, the previous results were confirmed with the worst performance in relation to the lowest mean of neoformed bone tissue for the CP group of 9.56mm3 , CB with 11.85mm3 and BG 14.247mm3 (p< 0.05). In conclusion with the results obtained by this work, the membranes studied here had a good biological behavior, and all of them helped in the guided bone regeneration process and the fish collagen membrane performed well in bone osteopromotion(AU)

Prevalence of middle ear disease in Chilean natives and the impact of development over 14 years / Prevalência de doenças da orelha média em nativos chilenos e o impacto do desenvolvimento ao longo de 14 anos

Abstract Introduction The prevalence of middle ear disease and its risk factors have been the subject of multiple studies. High prevalence of middle ear disease has been described among North American natives, especially chronic otitis media. Such studies have not been carried out in South America. Objective To describe the prevalence of middle ear pathology and risk factors in native schoolchildren from southern Chile who belong to the Mapuche ethnic group, as well as the impact of socio-economic and demographic changes after 14 years of development. Material and methods Two otologic evaluations with an interval of 14 years were performed in schoolchildren with a percentage of indigenous population above 85%. Socioeconomic and demographic data were collected from national official statistical data. Results A total of 1067 schoolchildren were examined. Many described risk factors for ear pathology were found. An overall prevalence of 0.19% for tympanic membrane perforation, 5.6% for pars tensa retraction pockets, 1.5% for pars flaccida retraction pockets and 11.1% of otitis media with effusion was found. There were several socioeconomic improvements after 14 years. The difference between the prevalence of symptoms and the presence of otitis media with effusion was statistically significant (p < 0.001). Conclusions Despite the presence of several risk factors for middle ear disease, this study population showed a low prevalence of middle ear disease. The ethnic-racial factor seems to be a protective factor.

Resumo Introdução A prevalência de doenças da orelha média e seus fatores de risco já foram objeto de múltiplos estudos. A alta prevalência dessas doenças foi descrita entre os nativos da América do Norte, principalmente a otite média crônica. Tais estudos não foram feitos na América do Sul. Objetivo Descrever a prevalência de doenças da orelha média e fatores de risco em crianças de idade escolar, nativos do sul do Chile pertencentes à etnia Mapuche, bem como o impacto de mudanças socioeconômicas e demográficas após 14 anos de desenvolvimento. Método Foram feitas duas avaliações otológicas com intervalo de 14 anos em escolares com percentual da população indígena acima de 85%. Dados socioeconômicos e demográficos foram coletados a partir de dados estatísticos oficiais nacionais. Resultados Foram examinados 1.067 escolares. Foram encontrados diversos fatores de risco descritos para doença otológica. Foi encontrada uma prevalência geral de 0,19% para perfuração da membrana timpânica, 5,6% para retrações da pars tensa, 1,5% para retração da pars flácida e 11,1% de otite média com efusão. Houve várias melhorias socioeconômicas após 14 anos. A diferença entre a prevalência de sintomas e a presença de otite média com efusão foi estatisticamente significante (p < 0,001). Conclusões Apesar da presença de vários fatores de risco para doença da orelha média, a população estudada apresentou baixa prevalência da doença. O fator étnico parece ser protetor.

Eficácia das membranas não absorvíveis na regeneração óssea guiada: uma revisão de literatura / Effectiveness of membranes non-absorbible in guided bone regeneration: a literature review

Introdução: As alterações dimensionais do processo alveolar após extração dentária é uma conse quência natural e fisiológica indesejável, podendo dificultar a colocação de um implante dentário na posição desejada. Portanto, a Regeneração Óssea Guiada (ROG) tem um papel relevante na pre venção da perda óssea. Objetivo: Compreender a eficácia do uso de membranas não absorvíveis na ROG de alvéolos pós extração. Metodologia: A pesquisa foi realizada nas bases de dados Pubmed, Scielo e BVS (Biblioteca Virtual em Saúde), além da busca no Google Acadêmico. Não foi aplicado nenhum limite temporal, nem restrição de idioma, como forma de abranger a maioria quantidade de trabalhos sobre o tema. Foram excluídos estudos que se distanciavam do objetivo desta revi são, bem como estudos de casos. Resultados e discussão: A ROG se consolida como uma técnica de preservação óssea e o seu princípio se relaciona com a osteopromoção. A fim de melhorar a capacidade de formação de osso, as membranas têm sido utilizadas como barreiras que guiam a cicatrização óssea e auxiliando na preservação do osso alveolar. Considerações finais: O uso da membrana não reabsorvível é muito viável e promissor na ROG em alvéolos pós-extração, com evidência em sua vantagem de preservação alveolar significativa... (AU)

Introduction: Dimensional changes in the alveolar process after tooth extraction is an undesirable natural and physiological consequence, which may make it difficult to place a dental implant in the desired posi tion. Therefore, Guided Bone Regeneration (ROG) has an important role in preventing bone loss. Objective: To understand the effectiveness of the use of non-absorbable membranes in the ROG of extraction sock ets. Methodology: The research was carried out in the databases Pubmed, Scielo and BVS (Virtual Health Library), in addition to the Google Scholar search. No time limit or language restriction was applied, as a way to cover the majority of works on the topic. Studies that differed from the objective of this review were excluded, as well as case studies. Results and discussion: ROG consolidates itself as a bone preservation technique and its principle is related to osteopromotion. In order to improve bone formation capacity, membranes have been used as barriers that guide bone healing and assist in the preservation of alveolar bone. Final considerations: The use of the non-resorbable membrane is very feasible and promising in ROG in post-extraction sockets, with evidence of its significant alveolar preservation advantage... (AU)

Potencial de regeneração óssea guiada das membranas de colágeno porcino (Jason e Collprotect Straumann®): estudo histológico, histomorfométrico e Micro TC em defeitos ósseos de calvária de ratos / Potential for guided bone regeneration of porcine collagen membranes (Jason and Collprotect Straumann): histological, histomorphometric, and Micro CT study in bone defects of rat calvaria

A regeneração óssea guiada (ROG) tornou-se uma prática comum na Implantodontia e para sua realização, é necessário o uso de membranas que auxiliem neste processo. As membranas absorvíveis têm mostrado vantagens em relação às membranas não absorvíveis e entre as características mais relevantes das membranas absorvíveis estão: o aporte vascular, suporte mecânico do tecido ósseo e a não necessidade de um segundo estágio cirúrgico. Esse estudo teve como objetivo avaliar e comparar, por meio das análises histológica, histomorfométrica e Micro TC o potencial osteopromotor de duas membranas de colágeno porcino comercialmente disponíveis em defeitos críticos de calvária de ratos. Para o estudo foram utilizados 96 ratos Albinus Wistar, divididos em quatro grupos, sendo 24 animais para cada grupo: Grupos BG (BioGide®); JS (Jason®); CS (Collprotect®) e CG (Coágulo) analisados em quatro tempos experimentais, 7, 15, 30 e 60 dias. Os resultados mostraram um perfil inflamatório mais agressivo dos grupos JS e CS em relação ao grupo BG (p<005). O grupo JS, aos 60 dias apresentou um potencial osteopromotor satisfatório ao compara-lo com o grupo BG (p=0,193) e o grupo CS demonstrou o pior desempenho osteopromotor. Na análise tridimensional, os resultados anteriores foram confirmados com o pior desempenho em relação a menor média de tecido ósseo neoformado para o grupo CS de 84,901 mm², JS com 246,802 mm² e BG 319,834 mm² (p<0,05). Podemos concluir que apesar das membranas serem compostas pelo mesmo material, as diferentes áreas de obtenção, espessuras e técnicas de tratamento da membrana, podem interferir no seu comportamento biológico em relação á quantidade de osso neoformado e que o grupo CS apresentou os piores resultados quando comparado aos grupos JS e BG(AU)

Guided bone regeneration (ROG) has become a common practice in Implantology and for its realization, it is necessary to use membranes that assist in this process. Absorbable membranes have shown advantages over non-absorbable membranes and among the most relevant characteristics of absorbable membranes are vascular supply and mechanical support of bone tissue. This study aimed to evaluate and compare, through histological, histomorphometric, and Micro CT analyzes, the osteopromotive factor of two commercially available porcine collagen membranes in critical calvaria defects in rats. 96 Albinus Wistar rats were used for the study, divided into four groups, 24 animals for each group: BG Groups (BioGide®); JS (Jason®); CS (Collprotect®) and CG (Clot) analyzed in four experimental times, 7, 15, 30 and 60 days. The results showed a more aggressive inflammatory profile of the JS and CS groups in relation to the BG group (p <0.05). The JS group, at 60 days, presented a satisfactory osteopromotive factor when comparing it with the BG group (p = 0.193) and the CS group demonstrated the worst osteopromotive performance. In the three-dimensional analysis, the previous results were confirmed with the worst performance in relation to the lowest average total volume of newly formed bone for the CS group of 84,901 mm², JS with 246,802 mm² and BG 319,834 mm² (p<0,05). We can conclude that although the membranes are composed of the same material, the different areas of obtaining, thicknesses, and techniques of treatment of the membrane, can interfere in its biological behavior in relation to the amount of newly formed bone and that the CS group presented the worst results among the JS and BG group(AU)

Uso de membranas como alternativa para remoção de fármacos em efluentes de estações de tratamento de esgotos / Use of membranes as an alternative for pharmaceuticals removal in effluents from sewage treatment plants

Os sistemas de tratamento de esgotos sanitários no Brasil possuem, em geral, algumas deficiências em termos de remoção de contaminantes, principalmente nutrientes e compostos orgânicos sintéticos introduzidos no meio ambiente pelo ser humano. Um dos tipos de contaminantes emergentes mais comuns em esgotos sanitários são os fármacos, que as tecnologias de tratamento biológico não são projetadas para a remoção de tais compostos. Este trabalho teve como objetivo principal avaliar a eficiência da remoção de fármacos e cafeína em estações de tratamento de esgoto existentes (lagoas de estabilização e lodos ativados), em diferentes pontos do processo, além da inclusão de sistemas pilotos de membranas de ultrafiltração, nanofiltração e osmose reversa, como etapa de polimento. Neste sentido, o uso de membranas teve o objetivo de elevar a eficiência de remoção de diferentes compostos. Usando a técnica de cromatografia líquida de alta eficiência acoplada à espectrometria de massas (LC-MS/MS) foi possível estabelecer uma metodologia de análise de fármacos com limites de quantificação baixos de até 0,1 ppb. Dentre os fármacos de interesse, o clonazepam e a sinvastatina não foram identificados em nenhum ponto de amostragem. O paracetamol, cafeína e ibuprofeno foram encontrados no esgoto afluente, sendo completamente removidos pelo tratamento biológico, tanto nas lagoas, como no sistema de lodos ativados. Já a carbamazepina, fármaco reconhecidamente recalcitrante, não foi removida em nenhuma ETE, sendo o sistema de membranas com osmose reversa uma etapa importante para sua remoção. O atenolol apresentou comportamento diferente, com remoção elevada no sistema de lagoas e baixa remoção no sistemas de lodos ativados. Neste sentido, a membrana de nanofiltração demonstrou ser uma importante barreira para evitar contaminação de corpos d'água por esse fármaco. Por fim, a análise econômica indicou que a nanofiltração possui um custo operacional menor que a osmose reversa, contudo com menores níveis de remoção de fármacos e outros contaminantes.

The sanitary sewage treatment systems in Brazil have, in general, some deficiencies in terms of removal of contaminants, mainly nutrients and synthetic organic compounds introduced into the environment by humans. One of the most common types of emerging contaminants in sewage are pharmaceuticals, which biological treatment technologies are not designed to remove such compounds. The main objective of this work was to evaluate the efficiency of pharmaceuticals and caffeine removal in existing sewage treatment plants (stabilization ponds and activated sludge), at different points in the process, in addition to the inclusion of membrane pilot systems of ultrafiltration, nanofiltration and reverse osmosis, as a polishing step. In this sense, the use of membranes aimed to increase the removal efficiency of different compounds. Using the technique of high performance liquid chromatography coupled with mass spectrometry (LC-MS/MS) it was possible to establish a methodology for pharmaceuticals analysis with low quantification limit of up to 0.1 ppb. Among the drugs of interest, clonazepam and simvastatin were not identified at any sampling point. Paracetamol, caffeine and ibuprofen were found in the raw sewage, being completely removed by biological treatment, both in stabilization ponds and activated sludge system. Carbamazepine, a known recalcitrant pharmaceuticals, was not removed in any WWTP, and the reverse osmosis membrane system was an important step for its removal. Atenolol had a different behavior, with high removal in the stabilization pond system and low removal in activated sludge system. In this sense, the nanofiltration membrane proved to be an important barrier to prevent contamination of water bodies by this compound. Finally, the economic analysis indicated that nanofiltration has a lower operational cost than reverse osmosis, however with lower levels of pharmaceuticals and other compounds removal.

Evaluación del daño renal por cadmio en población expuesta a contaminación por éste en agricultores de Quila-Quila, Potosí / Evaluation of kidney damage by cadmium in a population exposed to its contamination in farmers from Quila-Quila, Potosí

El cadmio es un metal pesado presente en desechos mineros y otros, y tiene efectos deletéreos en la salud humana especialmente en el riñón en el que destruye células tubulares. En una investigación de la calidad del lecho de los ríos que reciben aguas residuales en el norte de Potosí, Bolivia, se reportó presencia de cadmio en papas regadas con esa agua en Quila-quila, una población rural de la zona. OBJETIVO: de este trabajo fue el de identificar posibles daños del cadmio en la salud de los pobladores de Quila-quila. MÉTODOS: para ello se realizó revisión clínica y análisis laboratorial clínico y químico de sangre y orina de los pobladores de Quila-quila, y se calculó la tasa de filtración glomerular (TFG) y se correlacionó ésta con indicadores de lesión glomerular. RESULTADOS: se encontraron niveles elevados de cadmio en sangre en algunas personas, y una correlación de la TFG con la concentración de cadmio en sangre y con indicadores de lesión de membrana glomerular. CONCLUSIONES: en conclusión, los pobladores de Quila-quila estuvieron contaminados por cadmio, el cual produjo lesión renal leve.

Cadmium is a heavy metal present in mining and other wastes, and has deleterious effects on human health especially in the kidney in which it destroys tubular cells. In an investigation of the quality of the river beds that receive wastewater in the north of Potosí, Bolivia, the presence of cadmium was reported in potatoes watered with these water in Quila-quila, a rural town in the area. OBJECTIVE: of this work was to identify possible damage from cadmium to the health of the inhabitants of Quila-quila. METHODS: for this, clinical review and clinical and chemical laboratory analysis of blood and urine from the inhabitants of Quila-quila, and the filtration rate was calculated glomerular (GFR) and this was correlated with indicators of glomerular injury. RESULTS: elevated levels of cadmium were found in the blood in some people, and a correlation of GFR with blood cadmium concentration and indicators of membrane injury glomerular. CONCLUSIONS: in conclusion, the inhabitants of Quila-quila were contaminated by cadmium, which caused mild kidney damage.

Valoración de enfermería en gestantes con ruptura prematura de membranas / Nursing assessment in pregnant women with premature rupture of membranes

El proyecto se realizó con el objetivo de describir la valoración de Enfermería por dominios según NANDA-Internacional, en gestantes con diagnóstico de RPM, ingresadas en el Servicio de Ginecología y Obstetricia del Hospital Provincial General Docente Riobamba, Ecuador, durante el periodo octubre 2018 - enero 2019. Se realizó un estudio descriptivo, con enfoque cuantitativo, de corte transversal; cuya población de estudio quedó constituida por 61 individuos. Los datos fueron recolectados mediante la aplicación de una entrevista estructurada a cada paciente y de la revisión de documentos (historias clínicas). Se observaron 8 dominios alterados. El 63% de las mujeres participantes tuvo insuficientes controles prenatales. En el 78.7% de los partos se reportó líquido amniótico de aspecto claro. El 73.7% de las gestantes presentó antecedentes de infección. Las mayores afectaciones fueron en los dominios referidos a sexualidad/reproducción y promoción de la salud. La mayoría presentó RPM entre las 39 y 40.6 semanas y la FCF normal. Predominaron las madres adolescentes que declararon no planificar su embarazo.

This research was carried out in order to describe the assessment of Nursing by domains according to NANDA-International in pregnant women with a diagnosis of PROM admitted to the Gynecology and Obstetrics Service of the Provincial General Hospital of Riobamba-Ecuador, during the period October 2018 - January 2019. A cross-sectional and descriptive study was carried out with a quantitative approach; whose study population was made up of 61 individuals. The data were collected by applying a structured interview to each patient and reviewing documents (medical records). 8 altered domains were observed. 63% of the participating women had insufficient prenatal controls. Clear-looking amniotic fluid was reported in 78.7% of deliveries. 73.7% of pregnant women presented a history of infection. The greatest affectations were in the domains referring to sexuality / reproduction and health promotion. The majority presented PROM between 39 and 40.6 weeks and normal FHR. Adolescent mothers who declared not planning their pregnancy predominated.

Beneficios maternos en el manejo conservador de ruptura prematura de membranas ovulares (R. P. M) en embarazadas entre las 34 y 37 semanas de gestación atendidas en el Hospital Bertha Calderón Roque, Managua, Nicaragua, Julio - noviembre 2019 / Maternal benefits in the conservative management of premature rupture of ovular membranes (R. P. M) in pregnant women between 34 and 37 weeks of gestation attended at Bertha Calderón Roque Hospital, Managua, Nicaragua, July - November 2019

Objetivo: Determinar los beneficios maternos fetales en el manejo conservador de ruptura prematura de membranas ovulares (R.P.M) en embarazadas entre las 34 y 37 semanas de gestación atendidas en el Hospital Bertha Calderón Roque, Managua, Nicaragua, julio - noviembre 2019. Diseño metodológico: Se realizó estudio descriptivo, retrospectivo, de corte transversal, con universo y muestra de 89 pacientes y sus 89 neonatos, con (R.P.M). La información fue tomada de los expedientes clínicos, basado en la construcción de 2 instrumentos que contenían preguntas relacionadas a datos sociodemográficos de las pacientes y antecedentes gineco obstétricos, los criterios para el manejo conservador de la RPM y los posibles elementos de los beneficios maternos y los beneficios fetales. La información fue procesada en sistema estadístico SPSS 21.0 para Windows, representada en tablas y gráficos. El análisis fue de frecuencia simple y porcentajes. Resultados: La edad gestacional de diagnóstico de la RPM fue de 24 a 29sg 66.3%, con 100% de bienestar fetal y sin malformaciones, sin criterios de Gibbs en un 98.9%, se encontró infecciones urinarias y vaginales, obesidad y diabetes en un 43.8%. En el 95.5% sin infección puerperal y la finalización del embarazo fue a las 37 semanas en 89.9% por vía vaginal 86.5%. El peso al nacer fue en 79.7% mayor a 2500 gramos, con recién nacidos sanos 96.6%, con apgar de 8 al minuto en 94.4% y 9 a los 5 minutos 97.8%, sin muerte perinatal en un 100%. Conclusión: Los beneficios maternos más evidentes con el manejo conservador fueron la reducción de incidencia de Cesáreas, prolongación del embarazo disminuyendo riesgo de hemorragia posparto, y disminución de infecciones puerperales lo que permitió mantener la prolongación del embarazo y de esta manera lograr los beneficios fetales, adecuado peso al nacer, con buen APGAR, sin complicaciones, infecciosas, metabólicas y respiratorias

A new species of Parotocinclus with reduced adipose fin (Loricariidae: Hypoptopomatinae), from the rio Jacuípe basin, Bahia State, Brazil

A new species of Parotocinclus, apparently endemic to the rio Jacuípe basin, Bahia State, Brazil, is described. The new species is distinguished from congeners, except P. bidentatus, P. cabessadecuia, P. dani, P. halbothi, P. muriaensis, P. pentakelis, P. seridoensis, and P. spilurus, by the presence of a reduced adipose fin. The new species differs from the aforementioned species by the absence of unicuspid accessory teeth, abdomen with broad naked areas between lateral and medial patches of plates, and snout tip completely covered by plates and odontodes. The adipose fin of the new species is restrict solely to the adipose-fin spine, lacking the adipose-fin membrane, a reduction pattern previously described to P. halbothi, a congener from rivers of the Amazonas and Marowijne basins.(AU)

Uma espécie nova de Parotocinclus, aparentemente endêmica da bacia do rio Jacuípe, Estado da Bahia, Brasil, é descrita. A espécie nova se distingue de suas congêneres, exceto P. bidentatus, P. cabessadecuia, P. dani, P. halbothi, P. muriaensis, P. pentakelis, P. seridoensis e P. spilurus, pela presença de nadadeira adiposa reduzida. A nova espécie difere das espécies mencionadas anteriormente pela ausência de dentição acessória unicúspide, abdômen com amplas áreas nuas entre o grupo de placas laterais e o grupo de placas medianas e ponta do focinho completamente coberta por placas e odontódeos. A nadadeira adiposa da nova espécie é restrita somente ao espinho, sem a membrana da adiposa, um padrão de redução descrito para P. halbothi, uma congênere de rios da bacia Amazônica e da bacia do rio Marowijne.(AU)

Long-term Outcomes of Vitrectomy Used to Treat Myopic Traction Maculopathy

PURPOSE: We investigated the long-term outcomes of vitrectomy with internal limiting membrane (ILM) peeling as treatment for myopic traction maculopathy (MTM).METHODS: The medical records of patients who underwent vitrectomy to treat MTM were retrospectively evaluated. We excluded patients who exhibited macular holes (MHs) or retinal detachment at the time of primary surgery. The best-corrected visual acuity (BCVA) and central foveal thickness (CFT) were analyzed preoperatively, at 3, 6, 12, and 24 months after surgery, and at the final visit. Complications including retinal detachment or an MH were noted during follow-up.RESULTS: Twenty-three eyes of 22 patients were enrolled. At the time of primary surgery, the mean patient age was 64.4 ± 11.1 years and the baseline mean logMAR BCVA and CFT, 0.67 ± 0.50 and 431.8 ± 159.5 µm, respectively. The mean follow-up period was 53.7 ± 19.3 months. The mean logMAR BCVAs at 3, 6, 12, and 24 months postoperatively and at the final visit were 0.42 ± 0.39 (p = 0.001), 0.41 ± 0.38 (p = 0.001), 0.39 ± 0.40 (p < 0.001), 0.42 ± 0.43 (p < 0.001), and 0.51 ± 0.47 (p = 0.016), respectively, thus significantly better than the baseline value. The mean CFT at 3, 6, 12, and 24 months postoperatively and at the final visit were 244.6 ± 72.3, 210.5 ± 79.1, 209.6 ± 91.6, 219.8 ± 93.9, and 217.7 ± 81.3 µm, respectively, thus significantly less than baseline (all p < 0.001). MTM resolved in 18 eyes (78.3%) after primary surgery, without any complication, and remained stable to the final visit.CONCLUSIONS: Vitrectomy with ILM peeling afforded favorable long-term efficacy and safety in MTM patients.

Comparision of Hyperreflective Foci after Treatment of Diabetic Macular Edema Patients between Intravitreal Injections

PURPOSE: To compare the outcomes in patients with diabetic macular edema (DME) treated with intravitreal dexamethasone implants and intravitreal bevacizumab injections.METHODS: A retrospective cohort study was designed using 66 patients with DME treated with intravitreal dexamethasone (n = 35; 35 eyes) and intravitreal bevacizumab (n = 31; 31 eyes). Post-treatment changes in hyperreflective foci in the inner and outer retina were characterized using optical coherence tomography, central macular thickness, outer limiting membrane, and photoreceptor inner segment-outer segment junctions. Visual acuities were analyzed 4 weeks after bevacizumab injections and 8 weeks after dexamethasone injections.RESULTS: Both groups showed a decrease in the number of hyperreflective foci after treatment: from 10.6 ± 11.8 to 6.3 ± 5.9 (p = 0.005) in the intravitreal dexamethasone implant group and from 11.6 ± 8.5 to 7.7 ± 6.7 (p < 0.001) in the intravitreal bevacizumab injection group. The mean central macular thickness in the dexamethasone group changed significantly from 586.8 µm to 297.7 µm after treatment and the visual acuity improved significantly from 0.33 logMAR to 0.38 logMAR after treatment (p < 0.001 and p = 0.018, respectively). The mean central macular thickness in the bevacizumab group showed a significant decrease from 467.1 µm to 353.2 µm after treatment (p < 0.001), but there was no significant change in the visual acuities: 0.34 logMAR to 0.32 logMAR after treatment (p = 0.464).CONCLUSIONS: Both intravitreal dexamethasone implants and bevacizumab treatments in patients with DME showed improved outcomes including a decrease in hyperreflective foci shown by optical coherence tomography.

Comparison of Visual Acuity and Retinal Thickness According to Membranectomy in Idiopathic Epiretinal Membrane

PURPOSE: To compare the visual acuity and retinal thickness in patients with an idiopathic epiretinal membrane (ERM) after vitrectomy and membranectomy using two different methods.METHODS: This retrospective observational study included 77 eyes (32 eyes in the diamond-dusted membrane scraper [DDMS] group, and 45 eyes in the intraocular forceps [IOF] group) of 77 patients with idiopathic ERM who underwent pars plana vitrectomy and membranectomy. The main outcome measures were best-corrected visual acuity (BCVA, logMAR) and mean retinal thickness.RESULTS: In the IOF group, the BCVA at postoperative 1 week was significantly lower than at baseline and had improved at 12 weeks after surgery. In the DDMS group, the BCVA at postoperative 1 week showed no significant difference at baseline and had improved at 4 weeks after surgery. The central macular thickness at postoperative 6 months was significantly lower than at baseline (all, p < 0.001); there was no significant difference between the two groups (p = 0.400). The postoperative macular thickness of the DDMS group was significantly lower than that of the IOF group in the inner inferior and outer inferior areas at postoperative 12 weeks and 4 weeks (p = 0.046 and p = 0.039, respectively). Five eyes of the DDMS group and 15 eyes of the IOF group developed cystoid macular edema, 14 eyes of that improved without treatment.CONCLUSIONS: In patients with ERM, the use of DDMS or IOF for vitrectomy and membranectomy both resulted in improved visual acuity and decreased mean retinal thicknesses.

Distinctive bone regeneration of calvarial defects using biphasic calcium phosphate supplemented ultraviolet-crosslinked collagen membrane

PURPOSE: To overcome several drawbacks of chemically-crosslinked collagen membranes, modification processes such as ultraviolet (UV) crosslinking and the addition of biphasic calcium phosphate (BCP) to collagen membranes have been introduced. This study evaluated the efficacy and biocompatibility of BCP-supplemented UV-crosslinked collagen membrane for guided bone regeneration (GBR) in a rabbit calvarial model.METHODS: Four circular bone defects (diameter, 8 mm) were created in the calvarium of 10 rabbits. Each defect was randomly allocated to one of the following groups: 1) the sham control group (spontaneous healing); 2) the M group (defect coverage with a BCP-supplemented UV-crosslinked collagen membrane and no graft material); 3) the BG (defects filled with BCP particles without membrane coverage); and 4) the BG+M group (defects filled with BCP particles and covered with a BCP-supplemented UV-crosslinked collagen membrane in a conventional GBR procedure). At 2 and 8 weeks, rabbits were sacrificed, and experimental defects were investigated histologically and by micro-computed tomography (micro-CT).RESULTS: In both micro-CT and histometric analyses, the BG and BG+M groups at both 2 and 8 weeks showed significantly higher new bone formation than the control group. On micro-CT, the new bone volume of the BG+M group (48.39±5.47 mm3) was larger than that of the BG group (38.71±2.24 mm3, P=0.032) at 8 weeks. Histologically, greater new bone area was observed in the BG+M group than in the BG or M groups. BCP-supplemented UV-crosslinked collagen membrane did not cause an abnormal cellular reaction and was stable until 8 weeks.CONCLUSIONS: Enhanced new bone formation in GBR can be achieved by simultaneously using bone graft material and a BCP-supplemented UV-crosslinked collagen membrane, which showed high biocompatibility and resistance to degradation, making it a biocompatible alternative to chemically-crosslinked collagen membranes.

The biofilm removal effect of MnO₂-diatom microbubbler from the dental prosthetic surfaces: In vitro study / 대한치과보철학회지

PURPOSE: The aim of this study is to evaluate the effectiveness of MnO₂-diatom microbubbler (DM) on the surface of prosthetic materials as a mouthwash by comparing the biofilm removal effect with those previously used as a mouthwash in dental clinic.MATERIALS AND METHODS: DM was fabricated by doping manganese dioxide nanosheets to the diatom cylinder surface. Scanning electron microscopy (SEM) was used to observe the morphology of DM and to analyze the composition of doped MnO₂. Stereomicroscope was used to observe the reaction of DM in 3% hydrogen peroxide. Non-precious metal alloys, zirconia and resin specimens were prepared to evaluate the effect of biofilm removal on the surface of prosthetic materials. And then Streptococcus mutans and Porphyromonas gingivalis biofilms were formed on the specimens. When 3% hydrogen peroxide solution and DM were treated on the biofilms, the decontamination effect was compared with chlorhexidine gluconate and 3% hydrogen peroxide solution by crystal violet staining.RESULTS: Manganese dioxide was found on the surface of the diatom cylinder, and it was found to produce bubble of oxygen gas when added to 3% hydrogen peroxide. For all materials used in the experiments, biofilms of the DM-treated groups got effectively removed compared to the groups used with chlorhexidine gluconate or 3% hydrogen peroxide alone.CONCLUSION: MnO₂-diatom microbubbler can remove bacterial membranes on the surface of prosthetic materials more effectively than conventional mouthwashes.

Success and survival rate of the implant with crestal sinus lift using S-reamer and gel-type graft material: A retrospective study by more 5-years follow check up / 대한치과보철학회지

PURPOSE: The purpose of this retrospective study was to evaluate the method using the S-reamer and gel-type graft material by the success rate and survival rate.MATERIALS AND METHODS: Implantation period was from 2008 to 2014, Follow check up year is 2019. There were 59 patients and 117 implants. All implants were placed in the posterior maxilla with the sinus lift. The patients population consisted of 34 men and 25 women, ranging from 19 to 75 years. The residual bone heights were from 1 mm to 6 mm. Sinus was perforated with S-reamer without membrane tearing and gel type bone graft material was used for membrane lifting and filling the space. all implants were placed simultaneously. Panoramic X-ray was taken. After 5 – 6 months healing period, final prostheses were restored. After more 5-years implant surgery, Panoramic X-ray was obtained and X-ray analysis and clinical examination were performed. Success criteria was referred to a Buser's success critera. All implants were classified to success implant, survival implant, failed implant. A success implant was satisfying success criteria, a survival implant was a implant that was acute infection with suppuration and bone loss, a failed implant was a implant that was mobile, removed.RESULTS: Five implants were removed, and 4 implants had infected with bone loss. Survival rate was 95.7% and success rate was 92.3%.CONCLUSION: This retrospective study presented that this method with S-reamer and gel-type graft material was a successful treatment without membrane tear in the condition of 1-6 mm residual bone height.

Plasma CD36 and Incident Diabetes: A Case-Cohort Study in Danish Men and Women

BACKGROUND: Membrane CD36 is a fatty acid transporter implicated in the pathogenesis of metabolic disease. We aimed to evaluate the association between plasma CD36 levels and diabetes risk and to examine if the association was independent of adiposity among Danish population.METHODS: We conducted a case-cohort study nested within the Danish Diet, Cancer and Health study among participants free of cardiovascular disease, diabetes and cancer and with blood samples and anthropometric measurements (height, weight, waist circumference, and body fat percentage) at baseline (1993 to 1997). CD36 levels were measured in 647 incident diabetes cases that occurred before December 2011 and a total of 3,515 case-cohort participants (236 cases overlap).RESULTS: Higher plasma CD36 levels were associated with higher diabetes risk after adjusting for age, sex and other lifestyle factors. The hazard ratio (HR) comparing high versus low tertile of plasma CD36 levels was 1.36 (95% confidence interval [CI], 1.00 to 1.86). However, the association lost its significance after further adjustment for different adiposity indices such as body mass index (HR, 1.23; 95% CI, 0.87 to 1.73), waist circumference (HR, 1.21; 95% CI, 0.88 to 1.68) or body fat percentage (HR, 1.20; 95% CI, 0.86 to 1.66). Moreover, raised plasma CD36 levels were moderately associated with diabetes risk among lean participants, but the association was not present among overweight/obese individuals.CONCLUSION: Higher plasma CD36 levels were associated with higher diabetes risk, but the association was not independent of adiposity. In this Danish population, the association of CD36 with diabetes risk could be either mediated or confounded by adiposity.

Reverse vaccinology approach for the identification and characterization of outer membrane proteins of Shigella flexneri as potential cellular- and antibody-dependent vaccine candidates